The Influences of pH on the Enzyme Reaction-Lab Report
...uffer, which was different in each cuvette, 0.5 mL of water, and 0.5 mL of substrate. We added the substrate to the cuvettes last. We started timing the reaction at once with a set five minute reaction time. We then added 5.0 mL of NaOH to each of the cuvettes. After that, we used a spectrophotometer to obtain the absorbance of NaOh. We put one of the control test tubes in the spectrophotometer and set it to zero by pressing the 0% ABS button. We had to make sure not to get fingerprints on the out side of the tube, it would mess the measurement. The spectrophotometer measures how much light passes through the cuvette, if there are fingerprints on it, the right amount of light will not pass through it. After zeroing out the control substance, we then put one of the experimental cuvettes with NaOH in it in the spectrophotometer and measured it. We then recorded the absorbance on a table. We repeated these steps for the next four cuvettes and recorded the information on the same table as shown below. Absorbance Reading at 410 ...