concentration of hydrogen peroxide
Aim: To investigate how the concentration of hydrogen peroxide effects the rate of reaction of an enzyme (catalase) Variables: These factors could effect the rate of reaction on an enzyme: • pH • Concentration • Temperature • Surface Area pH - Enzymes function at different pH values. ... This graph shows that the enzyme activity reacts best at pH7 (neutral) Concentration - In concentrated solution there are more collisions between each particle, so the reaction occurs more quickly. This graph shows that increasing the concentration increases the enzyme activity. ... Brief Outline I will test the effects of changing the level of concentration. For this variable I will use three different concentrations of hydrogen peroxide with catalase (enzyme). I will change the concentration whilst keeping the time, concentration of catalase and the volume of hydrogen peroxide constant. ... In my experiment the substrate was the hydrogen peroxide, the enzyme that we used was hydrogen peroxide and the product that was formed was oxygen and water. ... Increasing the concentration of the reactants and raising the temperature make more collisions and therefore more fruitful collisions which increases the rate of reaction. ... Concentration The more concentrated the reactants are the greater the rate of reaction. ... This causes more collisions and therefore increases the rate of reaction Prediction After looking at the background information I predict that the mass of the oxygen produced will be proportional to the concentration of the hydrogen peroxide which will also be proportional to the rate of reaction. I think that if I increase the concentration of hydrogen peroxide I will increase the number of collisions, which will increase the rate of reaction. Therefore I think that if I double the concentration of hydrogen peroxide I will double the number of collisions which will double the rate of reaction I think this will happen because in general, concentrated solutions react more quickly than dilutes solutions. ... Dilute solution of Concentrated solution of H202 H202 Conclusion Increasing the concentration of the reacting solution can speed up reaction rates. ... Looking back at my experiments and the results I collected I can see that the concentration of hydrogen peroxide increased so did the volume of oxygen produced. This happened because as the concentration of the hydrogen peroxide increased so did the number of particles and this lead to an increase in the number of successful collisions and therefore the reaction rate increased along with the volume of oxygen gas. ... However I could not prove the second part of my prediction which was, if I double the concentration of H202 it would double the number of collisions, which would double the rate of reaction. If I pull out some results from my tables I can see that the amount of oxygen produced for each concentration did not double but it did increase. ... 3 Analysing From looking at the calculations of the rate of reaction I can say that all three concentrations of hydrogen peroxide (1. ... In my experiment the hydrogen peroxide decreased and the oxygen rose. ... Overall the hydrogen peroxide with the concentration of 6% had the fastest reaction because it had more particles for collisions to be successful than the 1.5% and 3% concentration of hydrogen peroxide. ... 5% concentration of H202 had a slower reaction than the 3%. ... 5% concentration than the 3%, this means there would be less chances of collision which would reduce the rate of reaction. This goes the same for the 6% concentration, as the 3% had a slower reaction than the 6%. ... Safety • Safety glasses were used during my experiments harmful chemicals were used • The measuring cylinder was placed on the table and I bent down to measure the hydrogen peroxide • I used the syringe slowly and carefully because if I pressed too hard there would be too much pressure and the catalase would spill out • The catalase was heated in a water bath for safety Fair Test To make my tests fair, I will investigate how the concentration of hydrogen peroxide, effects the rate of reaction by changing the concentration and keeping all other factors constant. ... Time - by allowing each experiment to proceed for only 2 minutes (trial experiment - 1 minute because we will be testing the experiment for any problems) Concentration of - by using the same concentration of catalase (1%) throughout catalase all the experiments.