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Identification of mitotic chromosomes using a squash preparation and a commercial slide

This experiment was carried out with the main aim of distinguishing and categorising chromosomes into their different stages of mitosis in a garlic root tip, and also to perform a count of cell category on a commercial cell.

Methods
In order to use a light microscope to view chromosomal detail, a squash preparation performed on the tissue was essential. This was carried out using the meristematic tip of a garlic root.
A segment, approximately 1mm was cut from the root tip using a scalpel, and placed with tweezers on a microscope slide.


Approximate Word count = 359
Approximate Pages = 1.4
(250 words per page double spaced)
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Identification of mitotic chromosomes using a squash prepara

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