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The movement of DNA Samples through charged electrophoresis gel
By Charlie Meyers
Introduction:
Nucleic acid samples are often subjected to gel electrophoresis to characterize the size and number of different fragments in the sample. ... The samples are subjected to agarose gel electrophoresis (electrical current is passed through a buffer system and gel).
Electrophoresis has many uses like DNA fingerprinting for the FBI or judicial cases. Also the human genome project has to do with electrophoresis gel testing. ...
Separation of nucleic acid molecules of different size is done by forcing the molecules to migrate through the gel in the electrical field. The gel acts like a sieve to allow small molecules to migrate faster than larger molecules. The result is that molecules separate in the gel according to their relative size and shape.
This exercise will use gel electrophoresis to examine the fragments present in DNA samples. In this lab experience is gained in pouring gels, loading DNA samples, and visualizing DNA bands on the gels. ... The molecules will separate in the gel according to their size and shape. ...
Methods:
Materials needed:
one gel electrophoresis box with electrode cords
one gel former with comb (to form wells in gel)
power supply box
Bottle of TAE buffer
Bottle of Tri sautate EDTA buffer
Tubes of DNA samples
Ethidium bromide dye
micropipette (2 µl to 10 µl) with disposable plastic tips (yellow)
Set the comb in the groove at the black end of your gel tray.
Approximate Word count = 925
Approximate Pages = 3.7
(250 words per page double spaced)
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