eating disorders
...riment is designed to utilize column chromatography and to explore the different variations possible within this technique. Procedure: To begin this experiment a glass buret or chromatography column should be clamped vertically with an ungreased closed stopcock. A small plug of cotton should be inserted loosely into the bottom of the column. This layer should then be covered with enough sand to form an inch thick layer (Gilbert 179). About 25mL of pet ether should then be added. Next, a funnel should be placed on top of the column and slowly, 5g of dry neutral alumina should be added while tapping the column. A rubber mallet can be made by placing a pencil into a rubber stopper. Now, the walls of the tube should be rinsed with additional pet ether to make sure all the alumina is off the sides and this later should be covered with a 1cm layer of sand. The stopcock should be opened and the solvent should be drained into a flask until solvent level is just above the top of the alumina. The sample mixture can now be added (Gilbert 179). A sample of a one to one mix of fluorine and 9-fluorenone should be obtained and the melting point range determined. In a small test tube, about 0.1g of this mix should be placed into 0.5mL of dichloromethane. Using a Pasteur pipette this solution should be transferred directly to the top of the column. The stopcock should be opened and the liquid level should be drained until it is just above the top of the alumina. Approximately 1-2mL of fresh pet ether should be added to the column and again the liquid level should be allowed to drain to the top of the alumina level. Next, a buret should be filled with 20mL of fresh pet ether and opening the stopcock, the eluant should be collected into a flask. The process of chromatography can be followed by collecting a drop or two of eluant onto a watch glass with every 5mL that elutes from the column. When the solvent evaporates, any white solid present will be visible on the watch glass. This process will determine the endp...