The effect of temperature on membrane permeability in beetroot
...CE BUNSEN IS LIT 1. Use a cork borer to cut cylinders of fresh beetroot tissue. Place on a tile and cut into 42, 3 mm wide discs. 2. Place all the discs in a small beaker and wash under a running tap for at least 5 minutes. 3. Meanwhile, label the test tubes - 30„aC, 40„aC, 50„aC, 60„aC, 70„aC, 80„aC and 100„aC and use a graduated pipette to add 6 cm3 cold water to each. 4. Prepare a water bath using a large beaker, tripod and gauze and Bunsen burner. 5. Heat gently to 30„aC and remove the Bunsen burner. 6. Impale 6 beetroot discs on a mounted needle with space between each disc. 7. Place the discs on the needle in the water bath for exactly 1 min. Push off the discs and drop them into the test tube labelled 30„aC. 8. Heat the water bath gently to 40„aC and place 6 more discs on to the needle. Place the discs into the water bath for 1 min, then push the discs off and into the test tube labelled 40„a C. 9. Repeat the procedure for the other tubes. Leave the discs in the test tubes for...