DNA Technology
...ich will cut the plasmid open and allow a piece of foreign DNA to be inserted. Restriction enzymes cut along palindromic base pairs, and in doing so creates sticky ends, which are single strands of DNA at the end of restriction sites. This step is important because if the right restriction enzyme is not used, then it is possible to cut the plasmid in the wrong place and cut a necessary gene. The DNA is absorbed by the bacterium by transformation. After the DNA has been transformed into the bacterium, the plasmid must be sealed with the enzyme ligase. Now the bacteria are grown. As they multiply, the plasmids along with the foreign DNA are duplicated. After days of growth, bacterial colonies form, and it is time for the scientists to see which colonies contain the incorporated foreign DNA. This is where the first step is important. Scientists can use an antibiotic on the colonies, and all the colonies th...