effect of ph on catalase
...d.(5cm3) 2. Enzyme concentration: The concentration of catalase will be unknown but by using a fixed mass of potato, the concentration of the enzyme can be more constant.(3g) 3. pH: Buffer solutions of each pH to be tested keep the reaction system constant. Risk assessment:! Hydrogen peroxide: It is an oxidizer, which is not flammable but may start a fire if put in contact with organic materials, so keep the area well ventilated. The maximum concentration that is safe to use in the laboratory is 20 vol. Make sure eye protection and protective clothing is worn, as hydrogen peroxide is corrosive. In case of any solution getting in contact with the eyes, flush it out with copious amounts of water and seek medical assistance. Any contact with the skin should be avoided but in case there is any contact, wash it off immediately with plenty of water as burns may result. Spillages should be diluted down with lots of water and washed up immediately. Hydrogen peroxide should be stored in a clearly labelled dark bottle, as it decomposes quickly with the presence of light, in a cool place. pH buffer solutions: As the solutions are of various pHs between 4.4to 8.2 they are acidic and basic, so solutions can have corrosive or caustic properties. Wear eye protection and avoid contact with the skin. If there is any spillages, including on the skin or eyes, wash it off immediately with plenty of water, especially if any of the solutions come into contact with the eyes. Store the buffers in clearly labelled bottles. 1 100 cm3 gas syringe (with rubber tubing)c 1 boiling tube; 1 250 cm3 beaker. 1 100 cm3 beaker: 1 10 cm3 graduated pipette (with filler) 1 spatulaf thermometer; 1 2 cm3 plastic syringe 1 25 cm3 measuring cylinder. 1 stop clock 1 bung (with 2 holes for plastic syringe and to connect to gas syringe) Weber oppressed structuralism . 1 clamp stand 1 electronic scale minced potato 20ol. hydrogen peroxide solution pH buffer solutions (pH 4.4 5.2 6.5, 7.5 8.2) distilled water balance Method: The Aparatus was set up as stated above 1. warm water was used in a plastic bowl for a water bath of 35 degrees. 2. 3g of potato was weighed using an electronic scale. 3. the potato was placed into a conical flask. 4. 10cm3of pH buffer solution was added to the potato and swirled to mix. 5. The mixture was paced in the water bath until the temperature reached 35oC.A bung was placed in the flask to ensure no oxygen escaped. 6. The measuring cylinder was filled with water and inverted into a trough of water. It was held in place using a clamp. At this point it was not placed over the delivery tube. 7. 5cm3 of hydrogen peroxide was taken using a syringe and connected to the flask. The hydrogen peroxide was then injected. The syringe was left in the tube so that the oxygen given off did not escape through the tube. 8. immediately the measuring tube was placed over the deliver tube and the stopcock was started. 9. The volume of oxygen was measured every thirty seconds for five minutes. 10. the experiment was then repeated with fresh potato and different PHs Results Ph 4.4 Time (minutes) Volume of O2(cm3) 30 0 1.00 0 1.30 0 2.00 0 2.30 0 3.00 0 ...